Daniel Sabath
Associate Professor
Laboratory Medicine
Adjunct Associate Professor
Medical Genetics

Faculty Information

Biography
Education & Training: 
MD, PhD
University of Pennsylvania, 1989
Hematopathology Fellowship
University of Washington
Laboratory Medicine Residency
University of Washington
Contact
Phone: 
(206) 897-5215
Mailing Address: 

Box 359743
325 9th Ave.
Seattle, WA 98104

Research & Clinical Interests
Research Interests: 

Dr. Sabath’s current research focuses on detection and characterization of circulating tumor cells. In collaboration with a local startup company, RareCyte, Inc., he has validated their circulating tumor cell assay for clinical use and is currently investigating the application of this technology for evaluating expression of other biomarkers in circulating tumor cells.

Dr. Sabath is also involved in clinical research in hemoglobin disorders and coagulation testing.

Clinical Interests: 

Dr. Sabath directs the Hematology and Coagulation laboratories at the UW Medicine - Montlake campus and Harborview Medical Center. He also directs the Red Cell Disorders Laboratory and Special Coagulation laboratory, both located at Harborview Medical Center. In addition, Dr. Sabath is actively involved in the Molecular Hematopathology laboratory operations, with significant signout responsibilities in that area. Finally, Dr. Sabath serves as a hematopathology consultant for the Harborview anatomic pathology services.

Publications
Publications: 
  1. Samuelson Bannow B, Warad DM, Jones CG, Pechauer SM, Curtis BR, Bougie DW, et al. A prospective, blinded study of a PF4-dependent assay for HIT diagnosis. Blood 2021 Feb 25; 137(8):1082-1089. doi:10.1182/blood.2020008195. Pubmed PMID: 32898858
  2. Basit A, Prasad B, Estergreen JK, Sabath DE, Alade N, Veenstra DL, et al. A Novel LC-MS/MS Assay for Quantification of Des-carboxy Prothrombin and Characterization of Warfarin-Induced Changes. Clin Transl Sci 2020 Mar 9; 13(4):718-726. doi:10.1111/cts.12757. Pubmed PMID: 32004415
  3. Parakh RS, Sabath DE. Venous Thromboembolism: Role of the Clinical Laboratory in Diagnosis and Management. J Appl Lab Med 2018 Jul 28; 3(5):870-882. doi:10.1373/jalm.2017.025734. Pubmed PMID: 31639762
  4. Alvarez W, Lai LH, Grant SJ, Sabath DE, Dillon J. Burkitt lymphoma of the maxilla in a HIV positive male - Presentation and review of diagnostic laboratory tests. Oral Maxillofac Surg Cases 2019 Jul 3; 5(3):. doi:10.1016/j.omsc.2019.100113. Pubmed PMID: 33282691
  5. Gu TM, Garcia DA, Sabath DE. Assessment of direct oral anticoagulant assay use in clinical practice. J Thromb Thrombolysis 2019 Apr; 47(3):403-408. doi:10.1007/s11239-018-1793-0. Pubmed PMID: 30600427
  6. Murphy CH, Sabath DE. Comparison of Phenotypic Activated Protein C Resistance Testing With a Genetic Assay for Factor V Leiden. Am J Clin Pathol 2019 Feb 4; 151(3):302-305. doi:10.1093/ajcp/aqy142. Pubmed PMID: 30423028
  7. Sabath DE. The Next Generation in Detection of Leukemia-Associated Translocations. J Mol Diagn 2018 Sep 27; 21(1):16-18. doi:10.1016/j.jmoldx.2018.08.009. Pubmed PMID: 30268945
  8. Mehalick ML, Schmaling KB, Sabath DE, Buchwald DS. Longitudinal associations of lymphocyte subsets with clinical outcomes in chronic fatigue syndrome. Fatigue 2018 Jan 12; 6(2):80-91. doi:10.1080/21641846.2018.1426371. Pubmed PMID: 30112249
  9. Sabath DE. Molecular Diagnosis of Thalassemias and Hemoglobinopathies: An ACLPS Critical Review. Am J Clin Pathol 2017 Jul 1; 148(1):6-15. doi:10.1093/ajcp/aqx047. Pubmed PMID: 28605432
  10. Flanagan ME, Andeen N, Lieberman J, Freeburg J, Williams JR, Hoogestraat DR, et al. An unusual case of a young patient with Whipple's disease involving the central nervous system. Clin Neuropathol 2017 Jul/Aug; 36(4):199-204. doi:10.5414/NP301001. Pubmed PMID: 28502319
  11. Murphy CH, Jain S, Sabath DE, Chatta GS, Hess JR. Spur cell hemolytic anemia in liver failure. Transfusion 2016 Apr; 56(4):787-8. doi:10.1111/trf.13407. Pubmed PMID: 27079310
  12. Sabath DE, Bender MA, Sankaran VG, Vamos E, Kentsis A, Yi HS, et al. Characterization of Deletions of the HBA and HBB Loci by Array Comparative Genomic Hybridization. J Mol Diagn 2015 Nov 21; 18(1):92-9. doi:10.1016/j.jmoldx.2015.07.011. Pubmed PMID: 26612711
  13. Campton DE, Ramirez AB, Nordberg JJ, Drovetto N, Clein AC, Varshavskaya P, et al. High-recovery visual identification and single-cell retrieval of circulating tumor cells for genomic analysis using a dual-technology platform integrated with automated immunofluorescence staining. BMC Cancer 2015 May 6; 15:360. doi:10.1186/s12885-015-1383-x. Pubmed PMID: 25944336
  14. Blom A, Bhatia S, Pietromonaco S, Koehler K, Iyer JG, Nagase K, et al. Clinical utility of a circulating tumor cell assay in Merkel cell carcinoma. J Am Acad Dermatol 2013 Dec 31; 70(3):449-55. doi:10.1016/j.jaad.2013.10.051. Pubmed PMID: 24388423
  15. Zhao M, Schiro PG, Kuo JS, Koehler KM, Sabath DE, Popov V, et al. An automated high-throughput counting method for screening circulating tumor cells in peripheral blood. Anal Chem 2013 Feb 6; 85(4):2465-71. doi:10.1021/ac400193b. Pubmed PMID: 23387387
  16. Wu D, Sherwood A, Fromm JR, Winter SS, Dunsmore KP, Loh ML, et al. High-throughput sequencing detects minimal residual disease in acute T lymphoblastic leukemia. Sci Transl Med 2012 May 16; 4(134):134ra63. doi:10.1126/scitranslmed.3003656. Pubmed PMID: 22593176
  17. Chapuis AG, Thompson JA, Margolin KA, Rodmyre R, Lai IP, Dowdy K, et al. Transferred melanoma-specific CD8+ T cells persist, mediate tumor regression, and acquire central memory phenotype. Proc Natl Acad Sci U S A 2012 Mar 5; 109(12):4592-7. doi:10.1073/pnas.1113748109. Pubmed PMID: 22393002
  18. Schiro PG, Zhao M, Kuo JS, Koehler KM, Sabath DE, Chiu DT. Sensitive and high-throughput isolation of rare cells from peripheral blood with ensemble-decision aliquot ranking. Angew Chem Int Ed Engl 2012 Feb 22; 51(19):4618-22. doi:10.1002/anie.201108695. Pubmed PMID: 22359315
  19. Sankaran VG, Xu J, Byron R, Greisman HA, Fisher C, Weatherall DJ, et al. A functional element necessary for fetal hemoglobin silencing. N Engl J Med 2011 Sep 1; 365(9):807-14. doi:10.1056/NEJMoa1103070. Pubmed PMID: 21879898
  20. Sabath DE, Greisman HA. A multiplex approach to the molecular diagnosis of β-thalassemia. J Mol Diagn 2011 Jun 7; 13(4):369-70. doi:10.1016/j.jmoldx.2011.05.001. Pubmed PMID: 21704268
  21. Fromm JR, Sabath DE, Wood BL. Diagnostic usefulness of flow cytometry for immunophenotyping classical Hodgkin lymphoma. Am J Clin Pathol 2011 Jul; 136(1):157-8; author reply 158. doi:10.1309/AJCP45LUYQSOKRVE. Pubmed PMID: 21685044
  22. diSibio G, Gabor EP, Lopategui J, Sabath DE, Alsabeh R, Cole JM. Large-cell transformation of a composite lymphoma. Exp Mol Pathol 2010 Jul 15; 89(3):260-7. doi:10.1016/j.yexmp.2010.07.001. Pubmed PMID: 20621095
  23. Furmanczyk PS, Wolgamot GM, Kussick SJ, Sabath DE, Olerud JE, Argenyi ZB. Diagnosis of mycosis fungoides with different algorithmic approaches. J Cutan Pathol 2009 Jul 7; 37(1):8-14. doi:10.1111/j.1600-0560.2009.01289.x. Pubmed PMID: 19602072
  24. Corrie SR, Lawrie GA, Battersby BJ, Ford K, Rühmann A, Koehler K, et al. Quantitative data analysis methods for bead-based DNA hybridization assays using generic flow cytometry platforms. Cytometry A 2008 May; 73(5):467-76. doi:10.1002/cyto.a.20534. Pubmed PMID: 18338761
  25. Pagel JM, Lionberger J, Gopal AK, Sabath DE, Loeb K. Therapeutic use of Rituximab for sinus histiocytosis with massive lymphadenopathy (Rosai-Dorfman disease). Am J Hematol 2007 Dec; 82(12):1121-2. doi:10.1002/ajh.21024. Pubmed PMID: 17696200
  26. Juarez T, Isenhath SN, Polissar NL, Sabath DE, Wood B, Hanke D, et al. Analysis of T-cell receptor gene rearrangement for predicting clinical outcome in patients with cutaneous T-cell lymphoma: a comparison of Southern blot and polymerase chain reaction methods. Arch Dermatol 2005 Sep; 141(9):1107-13. doi:10.1001/archderm.141.9.1107. Pubmed PMID: 16172307
  27. Andrews J, Setran E, McDonnel L, Kussick S, Wood BL, Sabath DE. An evaluation of the cell-dyn 3200 for counting cells in cerebrospinal and other body fluids. Lab Hematol 2005; 11(2):98-106. doi:10.1532/LH96.04071. Pubmed PMID: 16024333
  28. Quigley JG, Yang Z, Worthington MT, Phillips JD, Sabo KM, Sabath DE, et al. Identification of a human heme exporter that is essential for erythropoiesis. Cell 2004 Sep 17; 118(6):757-66. doi:10.1016/j.cell.2004.08.014. Pubmed PMID: 15369674
  29. Schmechel SC, LeVasseur RJ, Yang KH, Koehler KM, Kussick SJ, Sabath DE. Identification of genes whose expression patterns differ in benign lymphoid tissue and follicular, mantle cell, and small lymphocytic lymphoma. Leukemia 2004 Apr; 18(4):841-55. doi:10.1038/sj.leu.2403293. Pubmed PMID: 14961037
  30. Hoover DR, Donnay A, Mitchell CS, Ziem G, Rose NR, Sabath DE, et al. Reproducibility of immunological tests used to assess multiple chemical sensitivity syndrome. Clin Diagn Lab Immunol 2003 Nov; 10(6):1029-36. doi:10.1128/cdli.10.6.1029-1036.2003. Pubmed PMID: 14607863
  31. Sabath DE, Cross ST, Mamiya LY. An improved method for detecting red cells with hemoglobin H inclusions that does not require glass capillary tubes. Clin Lab Haematol 2003 Apr; 25(2):87-91. doi:10.1046/j.1365-2257.2003.00502.x. Pubmed PMID: 12641611
  32. Kussick SJ, Wood BL, Sabath DE. Mature T cell leukemias which cannot be adequately classified under the new WHO classification of lymphoid neoplasms. Leukemia 2002 Dec; 16(12):2457-8. doi:10.1038/sj.leu.2402715. Pubmed PMID: 12454754
  33. Killeen AA, Leung WC, Payne D, Sabath DE, Snow K, Tsongalis GJ, et al. Certification in molecular pathology in the United States (Training and Education Committee, The Association for Molecular Pathology). J Mol Diagn 2002 Nov; 4(4):181-4. doi:10.1016/s1525-1578(10)60701-5. Pubmed PMID: 12411584
  34. Chang CC, Lorek J, Sabath DE, Li Y, Chitambar CR, Logan B, et al. Expression of MUM1/IRF4 correlates with clinical outcome in patients with B-cell chronic lymphocytic leukemia. Blood 2002 Aug 1; 100(13):4671-5. doi:10.1182/blood-2002-01-0104. Pubmed PMID: 12393648
  35. Cerosaletti KM, Morrison VA, Sabath DE, Willerford DM, Concannon P. Mutations and molecular variants of the NBS1 gene in non-Hodgkin lymphoma. Genes Chromosomes Cancer 2002 Nov; 35(3):282-6. doi:10.1002/gcc.10114. Pubmed PMID: 12353271
  36. Masood N, Russell KJ, Olerud JE, Sabath DE, Sale GE, Doney KC, et al. Induction of complete remission of advanced stage mycosis fungoides by allogeneic hematopoietic stem cell transplantation. J Am Acad Dermatol 2002 Jul; 47(1):140-5. doi:10.1067/mjd.2002.122188. Pubmed PMID: 12077596
  37. Sabath DE, Barcy S, Koelle DM, Zeh J, Ashton S, Buchwald D. Cellular immunity in monozygotic twins discordant for chronic fatigue syndrome. J Infect Dis 2002 Feb 28; 185(6):828-32. doi:10.1086/339194. Pubmed PMID: 11920301
  38. Yeo TC, Xia D, Hassouneh S, Yang XO, Sabath DE, Sperling K, et al. V(D)J rearrangement in Nijmegen breakage syndrome. Mol Immunol 2000 Dec; 37(18):1131-9. doi:10.1016/s0161-5890(01)00026-8. Pubmed PMID: 11451418
  39. Sabath DE, Wood BL, Kussick SJ. PCR methods for determining B cell clonality. J Mol Diagn 2000 Nov; 2(4):217-8. doi:10.1016/s1525-1578(10)60640-x. Pubmed PMID: 11232112
  40. Seth A, Markee J, Hoering A, Sevin A, Sabath DE, Schmitz JE, et al. Alterations in T cell phenotype and human immunodeficiency virus type 1-specific cytotoxicity after potent antiretroviral therapy. J Infect Dis 2001 Feb 1; 183(5):722-9. doi:10.1086/318816. Pubmed PMID: 11181148
  41. Sprouse JT, Werling R, Hanke D, Lakey C, McDonnel L, Wood BL, et al. T-cell clonality determination using polymerase chain reaction (PCR) amplification of the T-cell receptor gamma-chain gene and capillary electrophoresis of fluorescently labeled PCR products. Am J Clin Pathol 2000 Jun; 113(6):838-50. doi:10.1309/02M7-5JCC-YRTK-MGDR. Pubmed PMID: 10874885
  42. Sabath DE, Shim MH. Use of green fluorescent protein/Flp recombinase fusion protein and flow cytometric sorting to enrich for cells undergoing Flp-mediated recombination. Biotechniques 2000 May; 28(5):966-72, 974. doi:10.2144/00285rr02. Pubmed PMID: 10818704
  43. Reimann KA, O'Gorman MR, Spritzler J, Wilkening CL, Sabath DE, Helm K, et al. Multisite comparison of CD4 and CD8 T-lymphocyte counting by single- versus multiple-platform methodologies: evaluation of Beckman Coulter flow-count fluorospheres and the tetraONE system. The NIAID DAIDS New Technologies Evaluation Group. Clin Diagn Lab Immunol 2000 May; 7(3):344-51. doi:10.1128/CDLI.7.3.344-351.2000. Pubmed PMID: 10799444
  44. Sabath DF, Lin N, Sabath DE, Kaushansky K, Broudy VC. Tyrosine 462 of the membrane-proximal F'-G' loop of murine Mpl is not essential for high-affinity binding of thrombopoietin. Cytokine 2000 Feb; 12(2):127-33. doi:10.1006/cyto.1999.0531. Pubmed PMID: 10671297
  45. Wood BL, Andrews J, Miller S, Sabath DE. Refrigerated storage improves the stability of the complete blood cell count and automated differential. Am J Clin Pathol 1999 Nov; 112(5):687-95. doi:10.1093/ajcp/112.5.687. Pubmed PMID: 10549256
  46. Davis CL, Wood BL, Sabath DE, Joseph JS, Stehman-Breen C, Broudy VC. Interferon-alpha treatment of posttransplant lymphoproliferative disorder in recipients of solid organ transplants. Transplantation 1998 Dec 27; 66(12):1770-9. doi:10.1097/00007890-199812270-00035. Pubmed PMID: 9884275
  47. Sabath DE, Koehler KM, Yang WQ, Phan V, Wilson J. DNA-protein interactions in the proximal zeta-globin promoter: identification of novel CCACCC- and CCAAT-binding proteins. Blood Cells Mol Dis 1998 Jun; 24(2):183-98. doi:10.1006/bcmd.1998.0185. Pubmed PMID: 9642099
  48. Sabath DE, Koehler KM, Yang WQ. Structure and function of the zeta-globin upstream regulatory element. Nucleic Acids Res 1996 Dec 15; 24(24):4978-86. doi:10.1093/nar/24.24.4978. Pubmed PMID: 9016669
  49. Horwitz M, Sabath DE, Smithson WA, Radich J. A family inheriting different subtypes of acute myelogenous leukemia. Am J Hematol 1996 Aug; 52(4):295-304. doi:10.1002/(SICI)1096-8652(199608)52:4<295::AID-AJH9>3.0.CO;2-N. Pubmed PMID: 8701948
  50. Oda D, Persson GR, Haigh WG, Sabath DE, Penn I, Aziz S. Oral presentation of posttransplantation lymphoproliferative disorders. An unusual manifestation. Transplantation 1996 Feb 15; 61(3):435-40. doi:10.1097/00007890-199602150-00021. Pubmed PMID: 8610357

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